pTKA001
(Plasmid #164577)

• Purpose: Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression
• Depositing Lab: Robert Kirchdoerfer
• Publication: Bacterial expression plasmids for the production of SARS-CoV-2 nsp7 (unpublished)

Backbone

• Vector backbone: pET46
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: nsp7
• Alt name: non-structural protein 7
• Insert Size (bp): 249
• GenBank ID: MN988668.1
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 6xHis, Ek, TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• Supplemental Documents:
  • pTKA001.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry