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pKR21:SSX1p-mK2-Ex1-(5'D)-ID-BD4-S-(30bp 30%GC)-(linker1)-pA

Plasmid

#251279

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR73:SSX1p-mK2-Ex1-(5'D)-AFP BD-S(50bp, 30%GC)-(linker1)-pA

Plasmid

#251285

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR13:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S (1-30bp)-(linker1)-pA

Plasmid

#251271

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR15:SSX1p-mK2-Ex1-(5'D)-ID-BD1-S(50bp, 50%GC)-(linker1)-pA

Plasmid

#251273

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR17:SSX1p-mK2-Ex1-(5'D)-ID-BD2-S(50bp 30%GC)-(linker1)-pA

Plasmid

#251275

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR3:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S(50bp, 30%GC)-(linker1)-pA

Plasmid

#251264

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_MOR_WT_APEX2

Plasmid

#250759

Purpose

Mammalian cell expression of the wildtype mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(WT)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Promoter

UBC

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_WT_APEX2

Plasmid

#250766

Purpose

Mammalian cell expression of GLUT4

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(WT)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Article

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xControl

Plasmid

#245729

Purpose

Express 42xControl mRNA in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xControl mRNA

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5415

Plasmid

#229030

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5444

Plasmid

#231064

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5448

Plasmid

#231065

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5452

Plasmid

#231067

Purpose

Inactive tetO-10xHis-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-10xHis-p2 dead-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-10xHis-phi29 DNAP dead-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN13-Elen0616-Cber(ElDSM2243)

Plasmid

#225308

Purpose

Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cber

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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