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We narrowed to 7,272 results for: tet

Showing: 7121 - 7140 of 7272 results

  3. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Mutation
    Promoter
    RPBSA and TRE promoter
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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  5. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Article
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

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  8. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  11. Desmoplakin I no force control (F40-based)

    Plasmid
    #119187
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    truncation after aa1945
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119187-plasmid-map-sequence-id-230534

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  12. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107576
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107576-plasmid-map-sequence-id-214314

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  14. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  18. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  20. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

    Plasmid
    #233065
    Purpose
    Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Leucine 928 to Alanine; Methionine 929 to Alanine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233065-plasmid-map-sequence-id-464575

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Showing: 7121 - 7140 of 7272 results