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twinStrep_HA_aMFx1 (4a)

Plasmid

#238905

Purpose

Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_HA_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL3986

Plasmid

#204132

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, wild type

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL811

Plasmid

#204135

Purpose

Low-copy constitutively expressed sfGFP for E. coli

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL830

Plasmid

#204136

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, D4

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch with decreasing mutant at position 4 regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGY240

Plasmid

#239811

Purpose

Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

H3
H2B

Use

Fungal expression

Tags

EGFP and mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY245

Plasmid

#239812

Purpose

Express H3-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY203

Plasmid

#239802

Purpose

Express GUS in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

GUS

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY209

Plasmid

#239804

Purpose

Express mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY219

Plasmid

#239806

Purpose

Express H2B-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY220

Plasmid

#239807

Purpose

Express H3-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY224

Plasmid

#239808

Purpose

Express H2B-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY225

Plasmid

#239809

Purpose

Express H3-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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PB-HSPB-Cre

Plasmid

#235217

Purpose

Cre expression under the control of HSPB promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

HSPB-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-HSPmin-Cre

Plasmid

#235218

Purpose

Cre expression under the control of HSPmin promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

HSPmin-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-synHSPB'3-Cre

Plasmid

#235219

Purpose

Cre expression under the control of synHSPB'3 promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

synHSPB'3-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-synHSPB'2-Cre

Plasmid

#235220

Purpose

Cre expression under the control of synHSPB'2 promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

synHSPB'2-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-HSP16F-Cre

Plasmid

#235221

Purpose

Cre expression under the control of HSP16F promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

HSP16F-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-HSPB'1-Cre

Plasmid

#235222

Purpose

Cre expression under the control of HSP'1 promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

HSPB'1-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCambia1300-ba-ar

Plasmid

#226815

Purpose

Efficient screening of gene editing in plants (Basta resistance).

Depositor

Daisuke Miki

Article

Kong et al Hortic Res. 2025 Nov 5;12(11):uhaf196. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST6GalNAc2 WT

Plasmid

#239827

Purpose

Displays Fc-fusion hST6GalNAc2 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST6GalNAc2 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-62 of hST6GalNAc2 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST3Gal4 WT

Plasmid

#239825

Purpose

Displays Fc-fusion hST3Gal4 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST3Gal4 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-42 of hST3Gal4 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-pST3Gal1 WT

Plasmid

#239822

Purpose

Displays Fc-fusion pST3Gal1 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-pST3Gal1 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(CD94)-Fc-pST3Gal1 WT

Plasmid

#239821

Purpose

Displays Fc-fusion pST3Gal1 WT on mammalian cell surface via transmembrane domain of CD94

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(CD94)-Fc-pST3Gal1 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSCG-TM(DPP4)-Fc-hST6Gal1 WT

Plasmid

#239826

Purpose

Displays Fc-fusion hST6Gal1 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST6Gal1 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-89 of hST6Gal1 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Ntom-Venus-ssrA

Plasmid

#223688

Purpose

PhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

ssrA peptide

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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