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pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Multi-Janus-Spacer-1x_pKG1126

Plasmid

#246375

Purpose

Multi-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG1526

Plasmid

#246357

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG2942

Plasmid

#246346

Purpose

ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF37-2A-mCherry-BGH_pKG2943

Plasmid

#246347

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-mCherry from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF37-2A-TagBFP-WPRE_pKG3675

Plasmid

#246348

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_PGK-VP16-ZF37-2A-TagBFP-WPRE_pKG3958

Plasmid

#246349

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the hPGK promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-GIB-Cre-N-WPRE_pKG3943

Plasmid

#246352

Purpose

GIB-Cre Retrovirus expressing N-terminal giberellin inducible Cre

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

iCre-N229-L1-GID1-NLS

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-GIB-Cre-C-WPRE_pKG3944

Plasmid

#246353

Purpose

GIB-Cre Retrovirus expressing C-terminal giberellin inducible Cre

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

GAI-L1-iCre-230C-NLS

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-VP16-ZF37-2A-mCherry-BGH_pKG1501

Plasmid

#246355

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-VP16-ZF37-2A-TagBFP-BGH_pKG3223

Plasmid

#246356

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(79-bp-DIAL-YB_TATA)-mGL-BGH_pKG1523

Plasmid

#246329

Purpose

79-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787

Plasmid

#246327

Purpose

loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(27-bp-DIAL-YB_TATA)-mGL-BGH_pKG1524

Plasmid

#246328

Purpose

27-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFC482

Plasmid

#246439

Purpose

The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

CasE

Tags

NLS

Expression

Mammalian

Mutation

SapI domesticated

Promoter

EF-1a

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEV824

Plasmid

#246453

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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