We narrowed to 30,952 results for: Tec;

1. pCMV-BE4max-NRRH

   Plasmid

   #136918

   Purpose

   Expresses BE4max-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRRH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL3927 (pDonor(plasmid)_Pse)

   Plasmid

   #200905

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (pTarget NGS)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE4max-NRTH

   Plasmid

   #136919

   Purpose

   Expresses BE4max-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ER-NFAST

   Plasmid

   #233596

   Purpose

   Expression of NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   NFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-RAB-EKARev-NLS

   Plasmid

   #118476

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev-NLS
   

   Tags

   6xHIS, Nuclear localization signal (NLS), T7 tag …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. rnLbCas12a-PE2

   Plasmid

   #213746

   Purpose

   For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   rnLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, H759A, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA304

    Plasmid

    #216072

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P shSOD1

    Plasmid

    #102975

    Purpose

    Suppression of SOD1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD1 (SOD1 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Actin Binding Domain Tension Sensor

    Plasmid

    #215368

    Purpose

    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tension Sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.minBG-CI-SaCas9-W3SynpA

    Plasmid

    #231369

    Purpose

    minBG-driven SaCas9, with short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

    Plasmid

    #231370

    Purpose

    SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. [pSK467] pLJC5 HA-Depdc5

    Plasmid

    #109343

    Purpose

    Lenti-viral expression of HA-tagged wildtype Depdc5

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CaTCH BC1

    Plasmid

    #154195

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    CaTCH BC1
    

    Use

    Lentiviral; Catch barcode labeling

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaTCH_empty-backbone

    Plasmid

    #157746

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral; Catch barcode cloning

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA031

    Plasmid

    #216011

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpRY (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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