We narrowed to 43,429 results for: Ina

1. p667-UBC-ADCK2-V5-miniTurbo_IDG-K

   Plasmid

   #189908

   Purpose

   Gateway destination clone of ADCK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ADCK2 (ADCK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENT-L3-a-element-L4

   Plasmid

   #186366

   Purpose

   Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   a-element early Otx neural enhancer
   

   Use

   Protein expression

   Promoter

   T7 promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCL8

   Plasmid

   #176184

   Purpose

   gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. tcTRP24sub8_Cap

   Plasmid

   #175797

   Purpose

   de novo thick circular tandem repeat protein capped to prevent multimerization

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub8_Cap
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SH2-free

   Plasmid

   #175800

   Purpose

   Human Nck2 SH2 domain

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   human Nck2 SH2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. tcTRP9

   Plasmid

   #175793

   Purpose

   de novo thick circular tandem repeat proteins with 9 repeats

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP9
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-CLK3_IDG-K

   Plasmid

   #170579

   Purpose

   Gateway destination clone of CLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CLK3 (CLK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-miniTurbo-V5-EEF2K_IDG-K

    Plasmid

    #170592

    Purpose

    Gateway destination clone of EEF2K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-EEF2K (EEF2K Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD/His-D-iLight

    Plasmid

    #170270

    Purpose

    Expression of iLight with 6xHis N-terminal tag in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    araBAD

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TAC-KRAB-dCas9

    Plasmid

    #120355

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p663-UBC-miniTurbo-V5-CAMKV_IDG-K

    Plasmid

    #158170

    Purpose

    Gateway destination clone of CAMKV (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMKV (CAMKV Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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