We narrowed to 29,240 results for: pET

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HiFi SpCas9-NLS-6xHis

Plasmid

#207378

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-865)

Plasmid

#203335

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-865 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-885)

Plasmid

#203334

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-885 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a- phage MazG-like/Atd1

Plasmid

#195244

Purpose

Expersses phage MazG-like/Atd1

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage MazG

Tags

Flag and TwinStrep

Expression

Bacterial

Mutation

Catalytic site mutant

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET-20b_HtrA2(delta(133))_S306A_6His

Plasmid

#197975

Purpose

E. coli expression of HtrA2(delta133)-6His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

HtrA2 (HTRA2 Human)

Tags

6His

Expression

Bacterial

Mutation

delta(1-133), S306A

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-A602E_M

Plasmid

#146964

Purpose

Bacterial Expression of NcQde-2_506-938-A602E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-A602E

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-H609W_M

Plasmid

#146965

Purpose

Bacterial Expression of NcQde-2_506-938-H609W

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-H609W

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-K634A_M

Plasmid

#146966

Purpose

Bacterial Expression of NcQde-2_506-938-K634A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K634A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-R895A_M

Plasmid

#146967

Purpose

Bacterial Expression of NcQde-2_506-938-R895A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-R895A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-Y595L_M

Plasmid

#146968

Purpose

Bacterial Expression of NcQde-2_506-938-Y595L

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-Y595L

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-HsAGO2-448-859-optim_K

Plasmid

#146729

Purpose

Bacterial Expression of HsAGO2_448-859-optim

Depositor

Elisa Izaurralde

Insert

HsAGO2_448-859-optim (AGO2 Human)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmEDC3_331-400-ADA_H

Plasmid

#146447

Purpose

Bacterial Expression of DmEDC3_331-400-ADA

Depositor

Elisa Izaurralde

Insert

DmEDC3_331-400-ADA (Edc3 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO5(wt)

Plasmid

#191803

Purpose

Full length wt AtPCO5 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsXRN1_1174-1649-Strep_AH

Plasmid

#148901

Purpose

Bacterial Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3-C93A-C96A_rpoA-U

Plasmid

#190962

Purpose

Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190970

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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