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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,673 results for: yeast

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Showing: 7181 - 7200 of 10673 results

  1. pVL1710

    Plasmid
    #239543
    Purpose
    Express Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239543-plasmid-map-sequence-id-476623

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  2. pVL1708

    Plasmid
    #239542
    Purpose
    Express Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239542-plasmid-map-sequence-id-476621

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  3. pFA6a-psfGFP-kanMX6

    Plasmid
    #240551
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    psfGFP
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240551-plasmid-map-sequence-id-479473

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  4. pFA6a-pTagRFP-T-kanMX6

    Plasmid
    #240555
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    pTagRFP-T
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240555-plasmid-map-sequence-id-479472

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  5. pKS1612

    Plasmid
    #239530
    Purpose
    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239530-plasmid-map-sequence-id-475878

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  6. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

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  7. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

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  8. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

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  9. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

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  10. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

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  11. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

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  12. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

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  13. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Expression
    Yeast
    Mutation
    Intron truncation
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

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  14. pREP41xN_ccdB

    Plasmid
    #79023
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79023-plasmid-map-sequence-id-151952

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  15. pREP42xN_ccdB

    Plasmid
    #79024
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79024-plasmid-map-sequence-id-147781

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  16. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/mL of DAP
    Species
    Escherichia coli
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  17. pHflu1

    Plasmid
    #203878
    Purpose
    pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer
    Depositor
    Bogumil Karas
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203878-plasmid-map-sequence-id-408184

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  18. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  19. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Benjamin Glick
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Tags
    msYFP2
    Expression
    Yeast
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218969-plasmid-map-sequence-id-445656

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  20. pBHM2642

    Plasmid
    #229526
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229526-plasmid-map-sequence-id-459924

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Showing: 7181 - 7200 of 10673 results