We narrowed to 7,272 results for: tet

1. PB-TO-hNIL

   Plasmid

   #172113

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AspFlex Kit

   Plasmid Kit

   #1000000217

   Purpose

   This collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.

   Depositor

   Veronica Godoy-Carter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

3. Endy/Shearwin pOSIP plasmid set

   Plasmid Kit

   #1000000035

   Purpose

   11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements

   Depositor

   Drew Endy

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. MXS Chaining Kit

   Plasmid Kit

   #1000000064

   Purpose

   MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals

   Depositor

   Pierre Neveu

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

5. EcoFlex MoClo Extension, Volume I

   Plasmid Kit

   #1000000253

   Purpose

   Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

   Depositor

   Mikhail Shapiro

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

6. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. FX cloning Kit

   Plasmid Kit

   #1000000039

   Purpose

   Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems

   Depositor

   Eric Geertsma

   Available since

   April 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. sAJM.1506

   Bacterial Strain

   #108254

   Purpose

   Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

9. sAJM.1504

   Bacterial Strain

   #108251

   Purpose

   Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

10. sAJM.1505

    Bacterial Strain

    #108253

    Purpose

    Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

11. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christian Chalut, Christophe Guilhot

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

12. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

13. DH10beta F' DOT sbcC (donor strain)

    Bacterial Strain

    #12082

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Bacterial Resistance

    Tetracycline

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

14. LC-E02

    Bacterial Strain

    #78551

    Purpose

    E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

15. LC-E03

    Bacterial Strain

    #78552

    Purpose

    E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

16. HL 6692

    Bacterial Strain

    #69776

    Purpose

    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR

    Depositor

    Han Lim

    Article

    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

    Bacterial Resistance

    Chloramphenicol

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. HL 6693

    Bacterial Strain

    #69777

    Purpose

    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + Δdam

    Depositor

    Han Lim

    Article

    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

    Bacterial Resistance

    Chloramphenicol

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. HL 6730

    Bacterial Strain

    #69779

    Purpose

    MG1655 + LacIq at intS + ptet::YFP::Asp terminator at galK + KanR::pT7::T710::CFP at yfjv + ptet::OmpC::T7RNAP::CamR at yeeP + ΔOxyR + Δdam

    Depositor

    Han Lim

    Article

    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

    Bacterial Resistance

    Chloramphenicol

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

20. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only