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pFBD-BirA_WDR91:392-747

Plasmid

#210880

Purpose

Insect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204814

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro (orf1ab MERS)

Expression

Bacterial

Mutation

MSAVLQ/SGLVKM

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PABPC1_544-626_pGEX6P1

Plasmid

#209790

Purpose

Bacterial expression of PABPC1 Mlle domain

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

Insert

Mlle domain of PABPC1 (PABPC1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 544-626 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

Plasmid

#201466

Purpose

Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 111-136

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

Plasmid

#201467

Purpose

Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 137-160

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

Plasmid

#201468

Purpose

Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 202-230

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

Plasmid

#201469

Purpose

Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 287-316

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

Plasmid

#196878

Purpose

Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-Llgl1-WPRE-UbC-Emerald

Plasmid

#203805

Purpose

Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

Llgl1 (Llgl1 Mouse)
Emerald

Use

Lentiviral

Expression

Mammalian

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

Plasmid

#196876

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196886

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Dync1h1motor

Plasmid

#191332

Purpose

Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Dync1h1motor (Dync1h1 Mouse)

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAP14

Plasmid

#186262

Purpose

wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

wgaAB bicistronic
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-GFP_mmu-miR-292b-3p_sponge

Plasmid

#132622

Purpose

Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

Depositor

Constance Ciaudo

Insert

stop codon, mmu-miR-292b-3p sponge sequence and polyA signal

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRX-C751-E3616S

Plasmid

#182844

Purpose

mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRX (w Fly)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 186-199

Plasmid

#108285

Purpose

Expresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 186-199

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 144-178

Plasmid

#108287

Purpose

Expresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 144-178

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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