We narrowed to 37,056 results for: Spr

1. PX852

   Plasmid

   #62884

   Purpose

   C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_dCas9-1x7

   Plasmid

   #63601

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. bRA89

   Plasmid

   #100950

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lba_Cas13a_WT

   Plasmid

   #91867

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbaCas13a (LbaC2c2) WT
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330S-7

   Plasmid

   #58783

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB166

   Plasmid

   #86998

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLBH559_Tet-HisCas14a1Locus

   Plasmid

   #112502

   Purpose

   Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PL6-CS-WR

   Plasmid

   #122512

   Purpose

   construction for sgRNA transcription with Plasmodium falciparum U6 promotor

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   PfU6

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330A-1x3

    Plasmid

    #58767

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pN-PITCh-GFP

    Plasmid

    #127888

    Purpose

    Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330S-6

    Plasmid

    #58782

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. bRA90

    Plasmid

    #100951

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge