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pAP702e

Plasmid

#253945

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mNeonGreen:: SV40-NLS::Linker::Nucleoplasmin-NLS::mScarlet::Linker::T2A::Linker

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAP701e

Plasmid

#253942

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mScarlet::SV40-NLS::Linker::Nucleoplasmin-NLS::mNeonGreen::Linker::T2A::Linker

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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p416TEF1 Cystector (Cysteine biosensor)

Plasmid

#250654

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pRSETb 6xHis-Cystector (Cysteine biosensor)

Plasmid

#250652

Purpose

E. coli expression plasmid for 6xHisTag-Cystector (Cysteine biosensor)

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Ptet

Plasmid

#243925

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Plac

Plasmid

#243921

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Para

Plasmid

#243923

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, not including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PR

Plasmid

#243927

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Plac

Plasmid

#243910

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site. Lacks repressor, LacI.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PlacZYA without lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Para

Plasmid

#243912

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site. Lacks activator protein, AraC.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

ParaBAD without araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Ptet

Plasmid

#243914

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PR

Plasmid

#243916

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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EKAREN5-c

Plasmid

#246761

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (original YPet - mTurquoise-GL pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-c

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-y

Plasmid

#246762

Purpose

Control plasmid for EKAREN5 and EKAREN5-gl (synonymous variant YPet - mTurquoise2 pair)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-y

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF-cascade

Plasmid

#247069

Purpose

L-arabinose inducible E.coli cascade operon inserted in pCDF vector

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

E.coli Type I-E CRISPR cas

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin9

Plasmid

#239019

Purpose

Bacterial overexpression of de novo Rhodamine binder 9 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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DriD_78-327

Plasmid

#237853

Purpose

DriD missing the wHTH DNABD used to crystallize the apo protein

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 78-327

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo

Plasmid

#221725

Purpose

Expression of Zeocin resistance marker in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-dsRed

Plasmid

#217006

Purpose

lentiviral plasmid for gRNA cloning with selectable dsRed expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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