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lvl0-P-TRE3G

Plasmid

#229166

Purpose

lvl 0 promoter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

TRE3G

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5-Inert

Plasmid

#229168

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

inert

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-mKO2

Plasmid

#229183

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

mKO2

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-M2

Plasmid

#225735

Purpose

To express M2 (219-399) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis

Expression

Bacterial

Mutation

aa 219-399

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3K253E

Plasmid

#225739

Purpose

To express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3K253E

Tags

c-Myc

Expression

Bacterial

Mutation

igr3 K253E

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxNeon

Plasmid

#229942

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pZB260_pT7-STR-H3U3

Plasmid

#228506

Purpose

Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

HIS3/URA3 operon

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM_ΔndhD2.km

Plasmid

#185532

Purpose

a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

nptII gene flanked by ndhD2 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3WT

Tags

c-Myc

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-M1

Plasmid

#225734

Purpose

To express M1 (13-193) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis

Expression

Bacterial

Mutation

aa 13-193

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5c-cdl

Plasmid

#207649

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV100

Plasmid

#215306

Purpose

Expresses sfGFP under induction with the 3OC6-HSL Marionette Cassette (LuxR + PLuxB-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PLuxB

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV110

Plasmid

#215340

Purpose

Expresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PAcu

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV101

Plasmid

#215307

Purpose

Expresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PVanCC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV111

Plasmid

#215341

Purpose

Expresses sfGFP under induction with the Erythromycin Marionette Cassette (MphRAM + EryR + PMph-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PMph

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_sheep_catalytic domain

Plasmid

#214331

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-18_lemur

Plasmid

#214332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_lemur_catalytic domain

Plasmid

#214328

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_sheep

Plasmid

#214335

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rabbit

Plasmid

#214334

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_dog

Plasmid

#214336

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Caspase-4_rabbit_catalytic domain

Plasmid

#214330

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECB sgCASP9

Plasmid

#211525

Purpose

Deletes CASP9

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgFADD

Plasmid

#211527

Purpose

Deletes FADD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgRPS3

Plasmid

#211526

Purpose

Facilitate knock-in of RPS3-Keima fusion protein

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgRPS3

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCKL1

Plasmid

#211523

Purpose

Deletes UCKL1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCKL1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge