We narrowed to 23,366 results for: Nov;

1. pYZ_3WJrep_N10_switch

   Plasmid

   #132724

   Purpose

   T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_N10_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFX-GalT-SECFP

   Plasmid

   #174470

   Purpose

   Express SECFP with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with golgi apparatus-targeting sequence
   

   Use

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFX-Tom20-SECFP

   Plasmid

   #174185

   Purpose

   Express SECFP with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with mitchondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

   Plasmid

   #110561

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIhh742bp-Luc

   Plasmid

   #53605

   Purpose

   Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

   Depositor

   Xiangli Yang

   Article

   Wang et al Development. 2009 Dec;136(24):4143-53. d

   Insert

   Ihh promoter
   

   Use

   Luciferase; Reporter

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pFX-GalT-iRFP

   Plasmid

   #174474

   Purpose

   Express iRFP with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with golgi apparatus-targeting sequence
   

   Use

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIW086

   Plasmid

   #134132

   Purpose

   Bacterial expression of His-TEV-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-TEV-human Ska1 ; human Ska2
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K183A K184A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIhh-5A9-LUC

   Plasmid

   #46318

   Depositor

   Xiangli Yang

   Article

   Wang et al Development. 2009 Dec;136(24):4143-53. d

   Insert

   5 copies of A9 sequence from Ihh promoter
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   mouse osteocalcin gene 2 promoter

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. KTK_001

   Plasmid

   #180517

   Purpose

   Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYZ_3WJrep_N11_trigger

    Plasmid

    #132733

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N11_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Promoter

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E0040m (GFP)_CD

    Plasmid

    #66032

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KTK_247

    Plasmid

    #180529

    Purpose

    Entry vector containing pTac promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    pTac promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TA0474_pET21a(+)

    Plasmid

    #13839

    Depositor

    SeWon Suh

    Article

    Kang et al Acta Crystallograph Sect F Struct Biol C

    Insert

    IS200 transposase (Ta0474 Thermoplasma acidophilum)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUPER-CXCR4

    Plasmid

    #42052

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    CXCR4 shRNA (CXCR4 )
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPHR-EYFP

    Plasmid

    #103817

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP (CRY2 Mustard Weed)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ02B2Gm_AE

    Plasmid

    #66063

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIhh1.3K-Luc

    Plasmid

    #53604

    Purpose

    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pFX-GalT-Venus

    Plasmid

    #174472

    Purpose

    Express Venus with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFX-Tom20-Venus

    Plasmid

    #174187

    Purpose

    Express Venus with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. B0032m_BC

    Plasmid

    #66020

    Purpose

    MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJ02B2Gm_EF

    Plasmid

    #66064

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIW069

    Plasmid

    #134133

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CRISPR resistant

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIhh715bp-Luc

    Plasmid

    #53606

    Purpose

    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

27. beta4-nAChR (DPM negative control)

    Plasmid

    #86651

    Purpose

    expression of control shRNA

    Depositor

    Jaime Modiano

    Article

    Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

    Insert

    CHRNB4 (control)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

29. Ubiquitin (1-76; L71Y)

    Plasmid

    #110756

    Purpose

    Bacterial expression of human Ubiquitin (1-76; L71Y)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L71Y

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-hPMCA4b delta6

    Plasmid

    #47590

    Depositor

    Emanuel Strehler

    Article

    Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

    Insert

    PMCA4bdelta6 (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only