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pSEVA47_dCas9-GFP_AAN_4

Plasmid

#231415

Purpose

This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-X
sgRNA-Y

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIA036

Plasmid

#200425

Purpose

amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Insert

LacI

Tags

bsdronPA

Expression

Bacterial

Promoter

Ppen

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-St1Cas9-BlastR

Plasmid

#220976

Purpose

Expresses human codon-optimized St1Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

St1Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SlugCas9-HF-BlastR

Plasmid

#220973

Purpose

Expresses human codon-optimized SlugCas9-HF

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9-HF

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L(A830T)

Plasmid

#209693

Purpose

Expresses MAC3-tagged VPS35L(A830T) in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC3-tag

Expression

Mammalian

Mutation

A830T, changed Alanine 830 to Threonine

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Sa-SlugCas9-BlastR

Plasmid

#220968

Purpose

Expresses human codon-optimized Sa-SlugCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Sa-SlugCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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POC1478

Plasmid

#213804

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1476

Plasmid

#213802

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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CpX-1

Plasmid

#218676

Purpose

Expresses complexin-1 in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

Rat complexin-1 (Cplx1 Rat)

Tags

6 his

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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POC1477

Plasmid

#213803

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1475

Plasmid

#213801

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-H2B-MCS

Plasmid

#184041

Purpose

Cloning of GFP-H2B fusion proteins

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Type

Empty backbone

Tags

GFP and H2B

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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STREP-SUMO-FLAG-SeKaiC

Plasmid

#218179

Purpose

WT SeKaiC for phosphorylation/dephosphorylation study

Depositor

Andy LiWang

Article

Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

Insert

STREP-SUMO-FLAG-SeKaiC

Tags

STREP-Tag

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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