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pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab32 DN

Plasmid

#37739

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T33N (dominant negative)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab32 CA

Plasmid

#37740

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pET-51b(+)_His10x-TEV-Rho-tag

Plasmid

#249665

Purpose

Expression of rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAJE90-Ma-tfmW-RSA2

Plasmid

#251520

Purpose

Machinery plasmid expressing Methanomethylophilus alvus aaRS/tRNA for TAG suppression and incorporation of trifluoromethyl-tryptophan (tfm-Trp) into sfGFP150TAG in E. coli

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Bacterial

Promoter

Gln S

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightG2

Plasmid

#221670

Purpose

Expresses nLightG2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightG2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR2

Plasmid

#221671

Purpose

Expresses nLightR2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightR2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-SiR-tag

Plasmid

#249666

Purpose

Expression of silicon rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CD86-Rho-tag

Plasmid

#249669

Purpose

Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

CD86-Rho-tag

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SQLE_sg2

Plasmid

#244872

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SQLE_sg1

Plasmid

#244871

Purpose

Knockout of human SQLE

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SQLE sgRNA (SQLE Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST40-SIRT1

Plasmid

#242124

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

V5-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R DD-FAM98B

Plasmid

#211357

Purpose

Transient expression of DD-V5-FAM98B

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B (FAM98B Human)

Tags

DD-V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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arginine biosensor cpFLIPR

Plasmid

#205746

Purpose

Robust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp

Depositor

Colin Jackson

Article

Whitfield et al Protein Sci. 2015 Jun 9. doi: 10.1002/pr

Insert

cpFLIPR

Use

Unspecified

Tags

6 x Histidine purification tag (N-terminal), Biot…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA3

Plasmid

#238988

Purpose

Mammalian expression of bovine arrestin-3 short isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-3 short (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA2-3A

Plasmid

#238991

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I386A,V387A,F388A

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA2d7

Plasmid

#238989

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 174, 176, 177, 181-184

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-His(N)-SSU72

Plasmid

#231723

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

SSU72 (SSU72 Human)

Tags

6xHis-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-SEC63-FAPoptim

Plasmid

#221116

Purpose

FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim-SEC63

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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