We narrowed to 19,562 results for: Tec
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Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
ExpressionPlantPromoterpU6-26, pU6-29Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDB_182
Plasmid#216103PurposeCRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#155011PurposeExpresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cellsDepositorInsertSc++
ExpressionMammalianMutationT1227K, Loop Substitution from S. anginosusPromoterEF1ɑAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
gCH27 (crCD55-4)
Plasmid#217343PurposecrRNA targeting CD55DepositorAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-StAR1 (Retro)
Plasmid#222687PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianMutationPGK without BbsI cutsiteAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-lib 2.0
Plasmid#89638PurposesgRNA expression in mammalian cells after Gateway cloningDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterhU6Available SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CABE-T1.17
Plasmid#193260PurposeExpression of CABE-T1.17 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T1.17
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CABE-T2.9
Plasmid#193264PurposeExpression of CABE-T2.9 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T2.9
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205318PurposeAll-in-one AAV vector expressing ISDge10 TnpB and its reRNADepositorInserthumanized ISDge10 TnpB
UseAAVTags3 x HAExpressionMammalianAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-LMP1-IRES-GFP
Plasmid#220350PurposeExpresses EBV latent gene LMP1 in mammalian cellsDepositorAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CABE-T3.155
Plasmid#193270PurposeExpression of CABE-T3.155 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T3.155
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
TagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …Available SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …PromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0
Plasmid#164606PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVMutationS383TPromoterhSynAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
TrHKII-pGFPN3
Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
TagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…Available SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits