We narrowed to 27,074 results for: LEA

1. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Tags

   EGFP tag

   Expression

   Mammalian

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac_human-IL-18

   Plasmid

   #214338

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2T-CMV-ABEmaxCP1041-BlastR

   Plasmid

   #152990

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   ABE7.10-nSpCas9 CP1041
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

   Plasmid

   #84604

   Purpose

   Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

   Depositor

   James Chen

   Article

   Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

   Insert

   mCherry
   

   Use

   Zebrafish transgenesis

   Promoter

   8xGliBS-delta-crystallin minimal promoter

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-GRAB_HA1m

   Plasmid

   #190474

   Purpose

   Express the medium-affinity histamine sensor GRAB_HA1m in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

   Insert

   Histamine sensor GRAB_HA1m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti GFAABC1D-dCas9-KRAB-MeCP2

   Plasmid

   #194701

   Purpose

   Expresses dCas9-KRAB-MeCP2 in Astrocytes

   Depositor

   Timothy Jarome

   Article

   Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

   Insert

   GFAABC1D promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   None

   Promoter

   GFAABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human SERCA3 (pMT2)

   Plasmid

   #75189

   Purpose

   Mammalian expression of ATP2A3

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

   Insert

   ATP2A3 (ATP2A3 Human)
   

   Expression

   Mammalian

   Promoter

   adenovirus major late promoter

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHA-mLRH-1

   Plasmid

   #16342

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

   Insert

   LRH-1 (Nr5a2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

9. p2T-CMV-BE4maxCP1028-BlastR

   Plasmid

   #152993

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9 CP1028-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stx1a-12aa-mCer

    Plasmid

    #53236

    Purpose

    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    Syntaxin 1A (Stx1a Rat)
    

    Tags

    12aa-linker and mCerulean

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-EF1a-PTDSS1-IRES-Puromycin

    Plasmid

    #134667

    Purpose

    Expresses EGFP-tagged Mitochondria-associated ER membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    PTDSS1 (PTDSS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-Vinculin-N-21

    Plasmid

    #54304

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

    Insert

    Vinculin (VCL Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pGL3-p300 reporter

    Plasmid

    #11726

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    p300 promoter and 5' UTR (EP300 )
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CMV-CDAmax-BlastR

    Plasmid

    #152995

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    CDA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECFP-histone H3

    Plasmid

    #179698

    Purpose

    visualization of nucleus/chromosome

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Histone H3 (H3c10 Mouse)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-RosaL6 ELD mutations

    Plasmid

    #37198

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to Rosa L6 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs L6, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q486E, I499L and N496D

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL90003

    Plasmid

    #68262

    Purpose

    Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6 (Triticum aestivum)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29_LuxSit_i

    Plasmid

    #194299

    Purpose

    Bacterial expression of the de novo luciferase, LuxSit-i

    Depositor

    David Baker, Andy Yeh

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Expression

    Bacterial

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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