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Showing: 721 - 740 of 23743 results

  3. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid
    #70651
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. lentiCRISPR - C16orf80 sgRNA 2

    Plasmid
    #70650
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. lentiCRISPR - C3orf17 sgRNA 2

    Plasmid
    #70653
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C3orf17
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  17. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid
    #221566
    Purpose
    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 16nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221566-plasmid-map-sequence-id-437904

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  18. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

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Showing: 721 - 740 of 23743 results