We narrowed to 7,519 results for: lenti crispr

1. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3-Nm2Cas9-BlastR

   Plasmid

   #220980

   Purpose

   Expresses human codon-optimized Nm2Cas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   Nm2Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-CjCas9-BlastR

   Plasmid

   #220979

   Purpose

   Expresses human codon-optimized CjCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   CjCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-St1Cas9-BlastR

   Plasmid

   #220976

   Purpose

   Expresses human codon-optimized St1Cas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   St1Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.3-SlugCas9-HF-BlastR

   Plasmid

   #220973

   Purpose

   Expresses human codon-optimized SlugCas9-HF

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SlugCas9-HF
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3-Sa-SlugCas9-BlastR

   Plasmid

   #220968

   Purpose

   Expresses human codon-optimized Sa-SlugCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   Sa-SlugCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-ABE7.10-P2A-Puro

   Plasmid

   #112674

   Purpose

   Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

   Depositor

   Lukas Dow

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti MS2-P65-HSF1_Hygro

   Plasmid

   #61426

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Insert

   MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1A

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuideB3GNT5-neo

   Plasmid

   #220867

   Purpose

   encodes CRISPR sgRNA targeting human B3GNT5

   Depositor

   Jennifer Kohler

   Insert

   B3GNT5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

    Plasmid

    #138460

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-KRAB-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

    Plasmid

    #138461

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-VP64-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid

    #192665

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid

    #192666

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid

    #157985

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB700 lenti gRNA KanR neo_TtoC and hairpin extension

    Plasmid

    #167925

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti SYN-FLAG-dCas9-VPR

    Plasmid

    #114196

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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