We narrowed to 29,575 results for: ple

1. plex_306-ccdB-g418

   Plasmid

   #201133

   Purpose

   pLEX_306 plasmid with g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. plex_306-ccdB-zeo

   Plasmid

   #201134

   Purpose

   pLEX_306 plasmid with zeo marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRUPATH Triple Ga12

   Plasmid

   #196061

   Purpose

   Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha12-RLuc8 (GNA12 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307-TMPRSS2-puro

   Plasmid

   #158457

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-pHluorin_M153R-CD63

   Plasmid

   #172117

   Purpose

   Fluorescent reporter for CD63-positive exosome secretion

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   pHluorin_M153R in a small extracelluar loop of CD…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Puro-sgNT

   Plasmid

   #138681

   Purpose

   Expresses a non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6-H2B-mCherry

   Plasmid

   #89766

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   Histone H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2-Nanoluc-ccdB

    Plasmid

    #87078

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. plex_307-ccdB-hygro

    Plasmid

    #201143

    Purpose

    pLEX_307 plasmid with hygro marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. plex_307-ccdB-g418

    Plasmid

    #201141

    Purpose

    pLEX_307 plasmid with g418 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CSII-PPM1H-mApple

    Plasmid

    #198473

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-mApple (PPM1H Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

    Plasmid

    #214926

    Purpose

    Expresses presynaptic non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).cpsfGFP.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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