We narrowed to 8,732 results for: Set

1. pBV65

   Plasmid

   #108363

   Purpose

   A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

   Depositor

   Scott Moye-Rowley

   Article

   Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

   Insert

   loxP-natMX6-CgMET3prom-cre-loxP
   

   Use

   Gene disruption marker for candida glabrata

   Promoter

   C. glabrata MET3

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pv6_TAF3_2xPhd

   Plasmid

   #179399

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3_2xPhd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDM068

   Plasmid

   #216811

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Expression

   Bacterial

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-CAG-flex-MCS--4x6T

   Plasmid

   #196420

   Purpose

   Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pv6_CBX7_2xChromo

   Plasmid

   #179396

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   G113V in flexible linker

   Promoter

   CAGGS

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1-Neo-CAG-Flpe-ERT2

   Plasmid

   #68460

   Purpose

   AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   mammalian-codon-optimized Flpe recombinase fused with ERT2
   

   Use

   Synthetic Biology

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDual-IN-SMN1

   Plasmid

   #215721

   Purpose

   dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN1 EXON 7 (SMN1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDC32B-AtMIR390a-B/c

    Plasmid

    #51776

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pv6_MBD1_MBD

    Plasmid

    #179404

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1-pur-CAG-mCherry

    Plasmid

    #159458

    Purpose

    AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176628

    Purpose

    TF A self-activation cassette in MultiFate-2.3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pv6_CBX7_Chromo_TAF3_Phd

    Plasmid

    #179400

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZac2.1 U6-SaAi9L-U6-SaAi9R

    Plasmid

    #78604

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    gRNAs for SaCas9 targeting Ai9 locus
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1-Blasticidin-CAG-Flpe-ERT2

    Plasmid

    #68461

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-AtMIR390a-B/c

    Plasmid

    #51778

    Purpose

    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Gateway entry vector

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge