We narrowed to 171,419 results for: addgene

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pMTKM32

Plasmid

#233504

Purpose

Level 0 plasmid for MoClo, encodes inducible PGK1-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM02

Plasmid

#233474

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM04

Plasmid

#233476

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM06

Plasmid

#233478

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM08

Plasmid

#233480

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 R0+R1+R3 +SV40 base prom-GFP-IRES-AP

Plasmid

#225892

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

R0+R1+R3

Tags

EGFP

Expression

Mammalian

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA2-Filler

Plasmid

#237401

Purpose

For the sequential insertion of two guide RNAs into two U6gRNA cassettes.

Depositor

Zuoming Sun

Article

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Inserts

U6gRNA-1
U6gRNA-2
PGK-TagBFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

PGK promoter, U6 promoter, and U6, PGK

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-Dok1-mScarlet

Plasmid

#215535

Purpose

Expression vector with Dok1 tagged with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12(D199A,C231S)

Plasmid

#215518

Purpose

Dox-inducible lentiviral transfer vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGCS-3xHA-TMEM230

Plasmid

#225512

Purpose

TMEM230 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Tags

3xHA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-3xHA-TMEM230triplemut

Plasmid

#225513

Purpose

TMEM230triplemut expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Tags

3xHA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-3xFlag-ATP11B

Plasmid

#225511

Purpose

ATP11B expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

ATP11B (ATP11B Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235326

Purpose

PiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235318

Purpose

Lentiviral expression of mRuby2 with 3' microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235327

Purpose

PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235268

Purpose

Lentiviral expression of ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235317

Purpose

Lentiviral expression of mRuby2 with intronic microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-PCNAL2 (pc3385)

Plasmid

#213140

Purpose

Expresses human PCNA miRFP670 tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2021 Dec 16;49(22):12

Insert

PCNA (PCNA Human)

Tags

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 Mini-enhancer +SV40 base prom-GFP-IRES-AP

Plasmid

#225893

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

VSX2 Mini-enhancer

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-MYADM-HA

Plasmid

#235241

Purpose

Lentiviral vector to generate HA-tagged human MYADM stable expressing cell line

Depositor

Jan Carette

Article

Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

Insert

MYADM (MYADM Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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