Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,128 results for: Met

Showing: 7401 - 7420 of 10128 results

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pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Publication

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Publication

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID1-Citrine

Plasmid

#126520

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Publication

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72K

Plasmid

#127683

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72K

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72K

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72K

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C18

Plasmid

#127678

Purpose

Bacterial expression of His-tagged OphA-delta C18

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C18

Tags

His8

Expression

Bacterial

Mutation

delta C18

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y63F

Plasmid

#127680

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y63F

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y63F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y63F

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y66F

Plasmid

#127681

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y66F

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y66F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y66F

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72A

Plasmid

#127682

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72A

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72A

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72A

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y76F

Plasmid

#127684

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y76F

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y76F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y76F

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OpHA-delta C6-Y98A

Plasmid

#127685

Purpose

Bacterial expression of His-tagged OpHA-delta C6-Y98A

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OpHA-delta C6-Y98A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98A

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y98F

Plasmid

#127686

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y98F

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y98F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98F

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Q172A

Plasmid

#127687

Purpose

Bacterial expression of His-tagged OphA-delta C6-Q172A

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Q172A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Q172A

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-W400A

Plasmid

#127688

Purpose

Bacterial expression of His-tagged OphA-delta C6-W400A

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-W400A

Tags

His8

Expression

Bacterial

Mutation

delta C6-W400A

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-P399A

Plasmid

#127689

Purpose

Bacterial expression of His-tagged OphA-delta C6-P399A

Depositor

James Naismith

Publication

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-P399A

Tags

His8

Expression

Bacterial

Mutation

delta C6-P399A

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FG121VDHFR_R166QTS

Plasmid

#91238

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42FG121 and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_R166QTS

Plasmid

#91240

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

R166Q and W22H

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_R166QTS

Plasmid

#91243

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31VDHFR_R166QTS

Plasmid

#91245

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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