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Showing: 1 - 20 of 21 results

  1. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  3. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.
    Depositor
    Mikhail Shapiro
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  4. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.
    Depositor
    John Heap
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.
    Depositor
    Anke Becker
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. Zinc Finger Consortium OPEN Accessory Reagent Set

    Plasmid Kit
    #1000000013
    Purpose
    Contains plasmids and strains used to engineer Zinc Finger Nucleases (ZFNs) via the Oligomerized Pool ENgineering (OPEN) method.
    Depositor
    Keith Joung
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.
    Depositor
    Keith Joung
    Available since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. STAR TALE Assembly Kit

    Plasmid Kit
    #1000000117
    Purpose
    These plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.
    Depositor
    Steve Pollard
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Dominic Esposito
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. Target Accelerator Genetic Pathway Reference Set

    Plasmid Kit
    #1000000105
    Purpose
    Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 21 results