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Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MetNI (E166Q in MetN)
Plasmid#78320PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
UseTags10x Histidine Tag and 6x Histidine TagExpressionBacterialMutationE166QPromoterT7 PromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmiRNA1-OE-METTL16-sgMETTL16-MUT1
Plasmid#196206Purposeoverexpress mutant METTL16DepositorInsertSynonymous mutation of METTL16 ORF (METTL16 Human)
UseLentiviralTagsExpressionMutationSynonymous mutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRNA1-OE-METTL16-sgMETTL16-MUT2
Plasmid#196202Purposeoverexpress METTL16DepositorInsertSynonymous mutation of METTL16 ORF (METTL16 Human)
UseLentiviralTagsExpressionMutationSynonymous mutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRNA1-OE-METTL16 F187G-sgMETTL16-MUT1
Plasmid#196201Purposeoverexpress METTL16DepositorInsertMETTL16 F187G (METTL16 Human)
UseLentiviralTagsExpressionMutationF187GPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRNA1-OE-METTL16 PP185/186AA-sgMETTL16-MUT1
Plasmid#196205Purposeoverexpress mutant METTL16DepositorInsertMETTL16 PP185/186AA (METTL16 Human)
UseLentiviralTagsExpressionMutationPP185/186AAPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MET
Plasmid#23889DepositorInsertMET (MET Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET
Plasmid#222482PurposeCo-expression of two genes in yeast with FLAG and MYC tags and MET15 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG103QDProCFPp303
Plasmid#15591DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG46QDProCFPp303
Plasmid#15589DepositorInserthtt 46Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG72QDProCFPp303
Plasmid#15590DepositorInserthtt 72Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRG01-MCAP_MET
Pooled Library#123361PurposepRG01 with barcoded MCAP_Met crRNA array library cloned in, targeting combinations of metastasis drivers.DepositorExpressionMammalianSpeciesSyntheticUseCRISPR and LentiviralAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse Metabolism Library
Pooled Library#163966PurposeLentiviral CRISPR library targets 2,918 mouse metabolic genes with ~18,000 guide RNAs. The library contains ~6 gRNAs per gene.DepositorUseCRISPR and LentiviralAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse metabolism knockout library
Pooled Library#160129PurposeCRISPR library targets mouse metabolic genesDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_U6_sgRNA_Mettl3C
Plasmid#165420PurposesgRNA construct for targeting Mettl3 C-terminusDepositorInsertPspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only