We narrowed to 59,446 results for: cloning

1. pTRV2-GG

   Plasmid

   #105349

   Purpose

   virus-induced gene silencing

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   TRV-RNA2(5')
   

   Use

   In planta gene silencing; viral

   Mutation

   BsaI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC234r-ccdB/TPK2

   Plasmid

   #176179

   Purpose

   yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   dropout cassette containing toxic genes ccdB and TPK2
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human Integrin beta 2

   Plasmid

   #8640

   Depositor

   Timothy Springer

   Article

   Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

   Insert

   Integrin beta 2 (ITGB2 Human)
   

   Expression

   Mammalian

   Available Since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

4. LentiV2-mU6-evopreQ1

   Plasmid

   #210190

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187922

   Purpose

   Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Tags

   3xFLAG

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187918

   Purpose

   Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Tags

   3xFLAG

   Promoter

   CMV Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO V5 DNAJB2b

   Plasmid

   #19524

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB2b (DNAJB2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19/N-eCFP

    Plasmid

    #183155

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pePEmax-pPuro

    Plasmid

    #215688

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PEmax
    

    Use

    Unspecified

    Promoter

    EFS promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAtC

    Plasmid

    #78097

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Basta resistance

    Depositor

    Jin-Soo Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NLS-HA

    Expression

    Plant

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. bdSUMO-TAT-GFP-HiBit

    Plasmid

    #170991

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-TAT-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. hGip-R

    Plasmid

    #14942

    Depositor

    Bernard Thorens

    Article

    Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

    Insert

    Gip-R (GIPR Human)
    

    Expression

    Mammalian

    Mutation

    Short form of the receptor

    Available Since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pPV-EF1a-EGxxFP-iP-A

    Plasmid

    #204624

    Purpose

    Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2Bc-T MBP_BoMoC(ed)_F753A_6xH

    Plasmid

    #185710

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO GFP DNAJB9

    Plasmid

    #19505

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB9 (DNAJB9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAtC

    Plasmid

    #78098

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

    Depositor

    Jin-Soo Kim, Sang-Gyu Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCPP3373

    Plasmid

    #128718

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopQ1-1
    

    Expression

    Bacterial

    Mutation

    Synonymous point mutation in the wobble position…

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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