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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,189 results for: LEA

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Showing: 7421 - 7440 of 12189 results

  3. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Publication
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  4. pEG585

    Plasmid
    #40192
    Depositor
    Geraldine Seydoux
    Publication
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S404A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  7. pHL836

    Plasmid
    #62827
    Purpose
    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    gfp
    ipex leader
    hfq leader
    Use
    Synthetic Biology
    Tags
    ompC leader and st7 RBS
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pHL1969

    Plasmid
    #62832
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    M8::ryhB leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::sodB leader
    Expression
    Bacterial
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL2107

    Plasmid
    #62841
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::micC leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::ompC leader
    Expression
    Bacterial
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pHL1954

    Plasmid
    #62831
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    M8::ryhB leader
    hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::sodB leader and st7 RBS
    Expression
    Bacterial
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid
    #223112
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223112-plasmid-map-sequence-id-447635

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  18. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid
    #223115
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223115-plasmid-map-sequence-id-447640

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  19. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid
    #223116
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223116-plasmid-map-sequence-id-447639

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  20. Epac-S-H328

    Plasmid
    #228244
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228244-plasmid-map-sequence-id-451838

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Showing: 7421 - 7440 of 12189 results