XE169 mouse Wnt-1-myc-pGEM4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16855||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2746
Vector typeXenopus Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13-Rev
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Linearize with Eco R1 and transcribe with T7 to make RNA.
Reference: This is the clone published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE169 mouse Wnt-1-myc-pGEM4 was a gift from Randall Moon (Addgene plasmid # 16855 ; http://n2t.net/addgene:16855 ; RRID:Addgene_16855)