We narrowed to 9,493 results for: pho;

1. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

   Plasmid

   #124278

   Purpose

   Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Mutation

   R201A

   Promoter

   AOX1

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

   Plasmid

   #107730

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-SRGAP2A-FBAR-mRFP

   Plasmid

   #168502

   Purpose

   Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Insert

   SRGAP2A-FBAR (SRGAP2 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP Kind2 S181E

   Plasmid

   #105312

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181E

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP Kind2 S181A

   Plasmid

   #105307

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181A

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe 3xFLAG mouse Bmf

   Plasmid

   #17243

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf (Bmf Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pGK-hMIGA2-DsRed

   Plasmid

   #192867

   Purpose

   Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   DsRed

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-GOLPH3

    Plasmid

    #21688

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Gateway entry clone

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pFLAG R106L-CPTP

    Plasmid

    #170744

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R106L

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG K60A-CPTP

    Plasmid

    #170743

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K60A

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-Ly6G-miR30

    Plasmid

    #163358

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMY-Ly6G-Rluc_miR

    Plasmid

    #163339

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MI-Luciferase

    Plasmid

    #75018

    Purpose

    Encodes the expression of Luciferase (with out any fluorescent reporter)

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2015 May 7;125(19):2968-73. doi:

    Insert

    firefly Luciferase
    

    Use

    Luciferase and Retroviral

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMY-Lyz2-GSG-mVenus

    Plasmid

    #163348

    Purpose

    Retroviral vector to express C-terminally mVenus-tagged murine Lyz2

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Lyz2 (Lyz2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMY-Lyz2-GSG-mTurquoise

    Plasmid

    #163347

    Purpose

    Retroviral vector to express C-terminally mTurquoise2-tagged murine Lyz2

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Lyz2 (Lyz2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pECE beta1 R760A

    Plasmid

    #50511

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

    Insert

    integrin beta1 (ITGB1 Human)
    

    Expression

    Mammalian

    Mutation

    R760A

    Promoter

    SV40

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pTH744-CEN-RLuc/slowmaxCFLuc

    Plasmid

    #38224

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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