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pPB-MPP8_Flag2-Blast

Plasmid

#175038

Purpose

Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Adrm1

Plasmid

#19417

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p21 shRNA1

Plasmid

#25868

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

p21 (Cdkn1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C

Plasmid

#53769

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644*

Promoter

CMV

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP C1-SPG20 SC domain

Plasmid

#218580

Purpose

Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

deleted amino acids 1-417

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HMGA2+GFP

Plasmid

#25405

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

polyadenylation signal lacking

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
mCardinal-Lysosomes-20

Plasmid

#56166

Purpose

Localization: Lysosome Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LAMP1

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pRSET mNG-GECO1

Plasmid

#201933

Purpose

Expresses mNG-GECO1 indicator in bacteria

Depositor

Robert Campbell

Article

Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

Insert

mNeonGreenGECO1

Tags

HIS tag

Expression

Bacterial

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Clathrin

Plasmid

#79997

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Clathrin (CLTB Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-N

Plasmid

#53767

Purpose

mammalian expression of flag-tagged ATR N-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal domain, aa 1-1409

Promoter

CMV

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Expression

Mammalian

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

Plasmid

#112004

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6s-P2A-mKate2

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Cyclin F

Plasmid

#236466

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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