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p54.mAldh1a1.flag.mSrtA-3M.wpre

Plasmid

#226771

Purpose

secretory SortaseA in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p54.mAldh1a1.flag.mSrtA-3M.wpre

Plasmid

#226771

Purpose

secretory SortaseA in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-LAP3

Plasmid

#217661

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-LAP3

Plasmid

#217661

Purpose

For protein purification from Ecoli

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAG157

Plasmid

#226746

Purpose

CRISPRi guide RNA against TOMM22

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM22 (TOMM22 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG157

Plasmid

#226746

Purpose

CRISPRi guide RNA against TOMM22

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM22 (TOMM22 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG207

Plasmid

#226738

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TIMM10 (TIMM10 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG207

Plasmid

#226738

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TIMM10 (TIMM10 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyA

Plasmid

#226249

Purpose

Production of unmodified ApyA peptide.

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Insert

His6_ApyA

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyA

Plasmid

#226249

Purpose

Production of unmodified ApyA peptide.

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Insert

His6_ApyA

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC51

Plasmid

#226779

Purpose

Expresses Drosophila UNC-104(A255V) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (A255V) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Alanine 255 to Valine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC51

Plasmid

#226779

Purpose

Expresses Drosophila UNC-104(A255V) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (A255V) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Alanine 255 to Valine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-AGG-NLS-EGFP

Plasmid

#214602

Purpose

express EGFP ; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

EGFP

Use

Unspecified

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-AGG-NLS-EGFP

Plasmid

#214602

Purpose

express EGFP ; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

EGFP

Use

Unspecified

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-A-V5-His

Plasmid

#222849

Purpose

E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-A

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b_AT118-A-V5-His

Plasmid

#222849

Purpose

E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-A

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge