We narrowed to 59,446 results for: cloning

1. p5E-smyhc1

   Plasmid

   #126748

   Purpose

   Zebrafish slow myosin heavy chain promoter. Clone 667

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   smyhc1 (smyhc1 Zebrafish)
   

   Use

   Multisite gateway entry clone

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO DNAJB1

   Plasmid

   #19468

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB1 (DNAJB1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQTEV-STMN1

   Plasmid

   #31326

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   stathmin 1/oncoprotein 18 (STMN1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PresentER-MSIIFFLPL (GFP)

   Plasmid

   #102946

   Purpose

   PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   MSIIFFLPL (Serpinf1 Mouse, Synthetic)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-PTD004

   Plasmid

   #34798

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   GTP-binding protein PTD004 (OLA1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIFR-3

   Plasmid

   #233295

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Promoter

   p14G

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC6A3

   Plasmid

   #132160

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

   Insert

   SLC6A3 (SLC6A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. bdSUMO-E5TAT-GFP-HiBit

   Plasmid

   #170993

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-E5TAT-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDT-sgRNA-XMAS-1x

   Plasmid

   #164413

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid

    #175507

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KTK_002

    Plasmid

    #180527

    Purpose

    Entry vector containing J23104 promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    J23104 promoter
    

    Expression

    Bacterial

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP3416

    Plasmid

    #128720

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopT1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Tags

    3x-FLAG

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. epi-ABEmax

    Plasmid

    #135974

    Purpose

    Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pQTEV-SCP2

    Plasmid

    #31336

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sterol carrier protein 2 (SCP2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCPP5060

    Plasmid

    #128716

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

    Insert

    shcN-hopN1
    

    Expression

    Bacterial

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK015

    Plasmid

    #105347

    Purpose

    Bacterial translocation fo proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Mutation

    BsaI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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