We narrowed to 7,673 results for: ski

1. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pV1326

   Plasmid

   #111435

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-TACC3-His6

   Plasmid

   #69106

   Purpose

   For bacterial expression of recombinant human TACC3 tagged with GST and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PDX1-2A-eGFP

   Plasmid

   #66964

   Purpose

   PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   pancreatic and duodenal homeobox 1 (PDX1 Human)
   

   Use

   Unspecified

   Tags

   2A-eGFP

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVG1

   Plasmid

   #111444

   Purpose

   Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3 T7 Jip1

   Plasmid

   #51699

   Purpose

   JIP-1 may suppress signal transduction by the JNK pathway"

   Depositor

   Roger Davis

   Article

   Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

   Insert

   Jnk interacting protein 1 (Mapk8ip1 Mouse)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSBtet-2xMARS-nGFP-puro

   Plasmid

   #205239

   Purpose

   DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Transposon

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   tight TRE promoter

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH-2xMARS-nGFP-puro

   Plasmid

   #205238

   Purpose

   Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Lentiviral

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs-Trib3

    Plasmid

    #131606

    Purpose

    expression of mouse Trib3 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Trib3 (Trib3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSC-ASCL1-IRES-Sox11

    Plasmid

    #182234

    Purpose

    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

    Depositor

    Chun-Li Zhang

    Article

    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

    Insert

    ASCL1-IRES-Sox11
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcdna3 T7 Jip1b

    Plasmid

    #52122

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid

    #24417

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Rs1
    

    Use

    Entry vector

    Tags

    FLAG and mCherry

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UUUUA-pHG165c

    Plasmid

    #170107

    Purpose

    Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    UUUUA
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MMMMA-pHG165c

    Plasmid

    #170108

    Purpose

    Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    MelR
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. XXXXA-pHG165c

    Plasmid

    #170110

    Purpose

    Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    XXXXA
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. XXXXA_Q182A_R287H-pHG165c

    Plasmid

    #170111

    Purpose

    Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    XXXXA_Q182A_R278H
    

    Expression

    Bacterial

    Mutation

    Q182A, R287H

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RRRAA-pHG165c

    Plasmid

    #170112

    Purpose

    Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    RRRAA
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge