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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-Alpha 49

Plasmid

#244910

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-Alpha 49

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV68

Plasmid

#244907

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV68

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3016

Plasmid

#232528

Purpose

Expression plasmid of LetA(T402A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA T402A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3017

Plasmid

#232529

Purpose

Genetic complementation plasmid of LetA(PqiA-ZnR) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA(PqiA-ZnRN/PqiA-ZnRC)

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3158

Plasmid

#232530

Purpose

Genetic complementation plasmid of LetB(R63A) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63A

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3159

Plasmid

#232531

Purpose

Genetic complementation plasmid of LetB(R63N) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63N

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3160

Plasmid

#232532

Purpose

Genetic complementation plasmid of LetB(R63D) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63D

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2865

Plasmid

#232514

Purpose

Expression plasmid of split-LetA with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV and StrepTagII

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223)]-[LetA2(224-427)-Stre…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2879

Plasmid

#232519

Purpose

Genetic complementation plasmid of LetA(ΔZnR-C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA ΔZnR-C

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2913

Plasmid

#232523

Purpose

Expression plasmid of LetA(ΔZnR-N) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA ΔZnR-N

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2914

Plasmid

#232524

Purpose

Expression plasmid of LetA(ΔZnR-C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA ΔZnR-C

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2921

Plasmid

#232525

Purpose

Expression plasmid of LetA(S364A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA S364A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3014

Plasmid

#232526

Purpose

Expression plasmid of LetA(D181A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA D181A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3015

Plasmid

#232527

Purpose

Expression plasmid of LetA(S321A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA S321A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2638

Plasmid

#232501

Purpose

Expression plasmid of of LetA with LetB(E854B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetB E854Amber

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2658

Plasmid

#232502

Purpose

Genetic complementation plasmid of LetA(C51A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA C51A

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2659

Plasmid

#232503

Purpose

Genetic complementation plasmid of LetA(C54A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA C54A

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2745

Plasmid

#232504

Purpose

Expression plasmid of of LetA(W162B) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA W162Amber

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2772

Plasmid

#232505

Purpose

Genetic complementation plasmid of LetA(ΔZnR-N) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA ΔZnR-N

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge