We narrowed to 32,902 results for: Eng
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Plasmid#186912PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)2-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-2xL
Plasmid#186908PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK035
Plasmid#180827PurposeT7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coliDepositorArticleInsertSwGdmB
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK036
Plasmid#180828PurposeT7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coliDepositorArticleInsertCnGdmA
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSB-FICAT_Payload-Esp3I
Plasmid#179380PurposeTransposon vector prepared for Esp3I golden gate cloning of TransgeneDepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc3_gRNA1
Plasmid#178746PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLm_gRNA1
Plasmid#178752PurposeExpression of a gRNA that targets next to PAM library, used for L. mobilis type I-E systemDepositorInserta gRNA that targets next to PAM library, used for L. mobilis type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc2_gRNA1
Plasmid#178740PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3137
Plasmid#179617PurposePunc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFPDepositorInsertGCaMP6
TagsRFPExpressionWormPromoterPunc-4Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyPromoterJ23119Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas7
Plasmid#170092Purposeexpresses E. coli type I-E Cas7DepositorInsertE. coli type I-E Cas7
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas8
Plasmid#170093Purposeexpresses E. coli type I-E Cas8DepositorInsertE. coli type I-E Cas8
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCse2
Plasmid#170094Purposeexpresses E. coli type I-E Cse2DepositorInsertE. coli type I-E Cse2
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA43
Plasmid#172944PurposepQE30 encoding Wzz mutant i191DepositorInsertShigella flexneri wzz i191 mutant gene
Tags6xHisExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA47
Plasmid#172947PurposepQE30 encoding Wzz mutant i255DepositorInsertShigella flexneri wzz i255 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA49
Plasmid#172948PurposepQE30 encoding Wzz mutant i81DepositorInsertShigella flexneri wzz i81 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only