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We narrowed to 7,684 results for: ef1a

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Showing: 7521 - 7540 of 7684 results

  7. pLV_PP1-mCitrine

    Plasmid
    #172472
    Purpose
    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    Use
    Lentiviral
    Tags
    mCitrine and prolactin signal sequence (cleaved p…
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172472-plasmid-map-sequence-id-342800

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  9. pGA1-EF1α-sfGFP150TAG-4xtRNA

    Plasmid
    #251517
    Purpose
    Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021
    Insert
    sfGFP150TAG
    Tags
    His6 and V5
    Expression
    Mammalian
    Mutation
    150 TAG (amino acid 150 mutated to stop codon)
    Promoter
    EF1alpha
    Available Since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    251517-plasmid-map-sequence-id-502878

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  10. L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

    Plasmid
    #244190
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244190-plasmid-map-sequence-id-489496

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  11. L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

    Plasmid
    #244189
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244189-plasmid-map-sequence-id-489495

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  12. L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreen

    Plasmid
    #244175
    Purpose
    Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA NTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, and NTEVp (75S) (TNFRSF1B Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    TNFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244175-plasmid-map-sequence-id-487911

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  18. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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  19. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

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  20. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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Showing: 7521 - 7540 of 7684 results