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pFP.R377

Plasmid

#138247

Purpose

Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTP728

Plasmid

#216374

Purpose

xNup358-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup358-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβεζ

Plasmid

#187353

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLCS111

Plasmid

#209332

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Gata3-T2A-H2B-eGFP donor

Plasmid

#113119

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata3 donor region including homology arms and T2A-H2B-eGFP

Use

Synthetic Biology

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMK469 (mAID-dTAG BSD)

Plasmid

#214395

Purpose

mAID-dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-dTAG-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P20

Plasmid

#120031

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pECFP-histone H3

Plasmid

#179698

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Histone H3 (H3c10 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGL-p27UTR

Plasmid

#20881

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p27 3'UTR (Cdkn1b Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pCX-Klf2

Plasmid

#66655

Purpose

For expression of mouse Klf2.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf2 (Klf2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1 RagA

Plasmid

#73031

Purpose

Expresses human RagA from a CMV promoter for stable expression in mammalian cells

Depositor

David Sabatini

Article

Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

Insert

rragA (RRAGA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P26

Plasmid

#120037

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

Plasmid

#182009

Purpose

CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

IgKchL_HA_STf_myc_PDGFRtmb

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-CoChR-mRuby2-ST

Plasmid

#109131

Purpose

Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CoChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUBQ10-moxMaple-GW

Plasmid

#209353

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRU319

Plasmid

#167686

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-BGHpA

Plasmid

#92161

Purpose

For lncRNA overexpression (in trans)

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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