Purpose
Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
Insert
ATP1A1 G8 sgRNA + user-specified sgRNA
Use
CRISPR; Prime editing
Promoter
Tandem U6 promoters
Available Since
Dec. 14, 2021
Availability
Academic Institutions and Nonprofits only