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Addgene

We narrowed to 10,134 results for: HAE

Showing: 741 - 760 of 10134 results
  1. pL0_AC-H4

    Plasmid
    #202122
    Purpose
    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Insert
    H4 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PhaR promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid
    #169652
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Insert
    PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid
    #108696
    Purpose
    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion
    Insert
    rnhB
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-terminal truncation (7aa) removing PIP box
    Promoter
    tac
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid
    #169487
    Purpose
    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).
    Depositor
    Insert
    PCMV-driven membrane localization
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pVH615

    Plasmid
    #169604
    Purpose
    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).
    Depositor
    Insert
    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pDUP2

    Plasmid
    #223517
    Purpose
    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac
    Depositor
    Inserts
    CoGFP
    CoGFP (second copy)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pL0_EF-Fld

    Plasmid
    #202181
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    Fld terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pL0_EF-P49

    Plasmid
    #202176
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    P49 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pL0-EF-54730

    Plasmid
    #202163
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    54730 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pL0_EF-glna

    Plasmid
    #202183
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Insert
    glna terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 741 - 760 of 10134 results