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SITS-EGFP-Bin1a

Plasmid

#176020

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1a

Plasmid

#176024

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-STIM1 WPRE

Plasmid

#236234

Purpose

AAV expression of mouse STIM1 internally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Stim1 (Stim1 Mouse)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1b

Plasmid

#176025

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET28(+)-NudC

Plasmid

#175022

Purpose

Purification of NudC enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

nudC

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-CreERT2-P2A-NeoR

Plasmid

#253966

Purpose

Lentiviral expression of tamoxifen-inducible CreERT2 with neomycin (G418) resistance (P2A-NeoR)

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

CreERT2

Use

Cre/Lox and Lentiviral

Promoter

UbC

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mPDZD8-3xFLAG (1-1147)

Plasmid

#253950

Purpose

Mammalian Expression of 3xFLAG-tagged mouse PDZD8

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

Pdzd8 (Pdzd8 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM1 WPRE

Plasmid

#236233

Purpose

AAV expression of mouse STIM1 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Stim1 (Stim1 Mouse)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1b

Plasmid

#176021

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4a-mCherry

Plasmid

#176010

Purpose

Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEHISSSO6206

Plasmid

#97065

Purpose

Expressing Sulfolobus solfataricus orf SSO6206 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO6206

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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