-
Plasmid#44024DepositorInsertI-SceI fused to mTREX2
UseTagsNLS-HAExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4A02
Plasmid#71021PurposeGateway entry cloneDepositorInsertUbe3a (Ube3a Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-4xDQNAT
Plasmid#128391PurposeIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sitesDepositorInsertProtein D
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3_4xUAS_CP-candidate_luc+
Plasmid#125154PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteinsDepositorInsert4 x upstream activating sequence (UAS)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PorA-4xDQNAT
Plasmid#128392PurposeIn vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sitesDepositorInsertPorA
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTc-4xDQNAT
Plasmid#128393PurposeIn vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin fragment C domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H08
Plasmid#70996PurposeGateway entry cloneDepositorInsertNap1 (Nap1 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ctrl
Plasmid#86380PurposeVector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_DUM1A01
Plasmid#71088PurposeGateway entry cloneDepositorInsertpnr (pnr Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1A07
Plasmid#71083PurposeGateway entry cloneDepositorInsertgrn (grn Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2A03
Plasmid#70878PurposeGateway entry cloneDepositorInsertElp3 (Elp3 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4D06
Plasmid#71048PurposeGateway entry cloneDepositorInsertmor (mor Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3E07
Plasmid#70987PurposeGateway entry cloneDepositorInsertTfIIS (TfIIS Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUG-IRES-mCherry
Plasmid#222109PurposeStart codon reporter (WT AUG)DepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
300_pETcon_SARS2_FLip
Plasmid#222230Purposeyeast surface display of the SARS-CoV-2 FLip variant RBDDepositorInsertSARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
297_pETcon_SARS2_Omicron-BA286
Plasmid#222231Purposeyeast surface display of the SARS-CoV-2 BA.2.86 variant RBDDepositorInsertSARS-CoV-2 BA.2.86 RBD (S Budding Yeast, SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
299_pETcon_SARS2_EG-5
Plasmid#222232Purposeyeast surface display of the SARS-CoV-2 EG.5 variant RBDDepositorInsertSARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly_spike-in
Plasmid#125151PurposeVector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cellsDepositorInsertD. pseudoobscura peak #47 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only