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Plasmid#183904Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-attB-PUb-GFP
Plasmid#183911PurposeattB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoterDepositorInsertGFP
UseTagsExpressionMutationPromoterAedes aegypti PUbAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#133077PurposeX. laevis expression vector. It will generate the human TRAAK channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
UseTagsExpressionMutationQ258KPromoterT7Available sinceOct. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 C207F
Plasmid#68829PurposeExpresses c-myc-tagged human RAD18 with C207F mutationDepositorInsertc-myc tagged human RAD18 with C207F mutation (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pRC/CMV
Plasmid#8514DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionMammalianMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTox-EGFPsite1_NGA-PAM (BPK754)
Plasmid#181747Purposep11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAMDepositorInsertccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
UseSelection plasmidTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I
Plasmid#146553PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorInsertHsSmg6-del36-576-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDB181
Plasmid#80692Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06DepositorInsertgEHEE_06
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) HIS-tag pET
Plasmid#8553DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDB267
Plasmid#80695Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHHH_06DepositorInsertgHHH_06
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB278
Plasmid#80694Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44DepositorInsertgHH_44
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB294
Plasmid#80693Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHEEE_02DepositorInsertgHEEE_02
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB199
Plasmid#80691Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06DepositorInsertgEHE_06
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB289
Plasmid#80690Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02DepositorInsertgEEHE_02
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB185
Plasmid#80688Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04DepositorInsertgEEH_04
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB285
Plasmid#80687Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04DepositorInsertgEEEH_04
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-Val15AlafsX11
Plasmid#24732DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationdeleted amino acids 44-62. deletion/frameshift…PromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(G124I)
Plasmid#130672PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
UseTagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, G124IPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgSORCS2-G2
Plasmid#118416PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2DepositorInsertgRNA SORCS2 Human (SORCS2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only