We narrowed to 34 results for: buc
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TypeCollection.... R., Alexander, J. R., Allen, S., Amster, A., Arbuckle, J., Ayala, A., Baker, P. M., . . . Tasic, B. ....org/10.1016/j.cell.2025.05.002 PMID: 40403729 Graybuck, L. T., Daigle, T. L., Sedeño-Cortés, A. E., Walker...j.neuron.2025.04.035 PMID: 40403704 Mich, J. K., Graybuck, L. T., Hess, E. E., Mahoney, J. T., Kojima, Y...
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CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might denote ...your search as follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998). FLPe’s performance...18614669. PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998.... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post... folding and blocks RepZ translation (Asano & Mizobuchi, 1998). This system keeps RepZ levels low even...doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...Examples include the LOV domains that bind flavin (Buckley et al., 2015) or the UnaG protein that binds bilirubin...protein." (1998): 509-544. PubMed PMID: 9759496. 3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...et al., 2017, Vormstein-Schneider et al., 2020, Graybuck et al., 2021, Lawler et al., 2022). It can also...342. https://doi.org/10.1016/j.omtm.2022.07.010 Graybuck, L. T., Daigle, T. L., Sedeño-Cortés, A. E., Walker....org/10.1016/j.neulet.2017.11.049 Mich, J. K., Graybuck, L. T., Hess, E. E., Mahoney, J. T., Kojima, Y... -
K. phaffii: Rising to the Occasion
TypeBlog Post...yeast can also give more protein bang for your media buck - it grows at higher cell densities than most of... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...Ásgrímsdóttir ES, Rahimi K, Gill KP, Frandsen S, Hvolbøl Buchholdt S, Chen M, Kjems J, Febbraro F, Denham M (2018... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...'s better for you to use cold block systems or buckets of ice. Try finding ways to intentionally set up... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical... -
Synthetic Biology - Overview
TypeCollection...Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...and business training. The money is a drop in the bucket against the funds needed to build a flower that... -
Plasmids 101: Degron Tags
TypeBlog Post...20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, et al. The dTAG system... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...of negative strand RNA viruses. References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...gene. Validated gRNAs are a great bang for your buck – they have been used successfully in experiments... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...also make them more attuned to the bang for their buck - make them demand more services based on what they're... -
Selecting Your Plasmid Purification Kit
TypeBlog Post... make sure you’re getting the best bang for your buck. If you’re unsure if a kit is a good option for ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post.... 6(5): p. 568-72. PubMed PMID: 10802714. 41. Vierbuchen, T., et al., Direct conversion of fibroblasts...