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Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-NT
Plasmid#184568PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-1
Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-NT
Plasmid#184838PurposeExpression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.DepositorInsertCo-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJK_His-SUMO-LbuCas13a
Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
UseTagsHis6 and bdSUMOExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
SKA974
Bacterial Strain#80403PurposeJT2-based E. coli host strain for optogenetic control of cell growth.DepositorBacterial ResistanceKanamycinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAsylum
Plasmid#24754DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBoPyV3-S23
Plasmid#62369PurposeComplete genome of BoPyV3 isolate S23DepositorInsertFull genome of BoPyV3 isolate S23
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoPyV3-S22
Plasmid#62368PurposeComplete genome of BoPyV3 isolate S22DepositorInsertFull genome of BoPyV3 isolate S22
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoPyV2b-S24
Plasmid#62357PurposeComplete genome of BoPyV2b isolate S24DepositorInsertFull genome of BoPyV2b isolate S24
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoPyV2a-S22
Plasmid#62356PurposeComplete genome of BoPyV2a isolate S22DepositorInsertFull genome of BoPyV2a isolate S22
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoPyV1-S22
Plasmid#62326PurposeComplete genome of BoPyV1 isolate S22DepositorInsertFull genome of BoPyV1 isolate S22
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only