We narrowed to 18 results for: buc

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might denote ...your search as follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes...

2. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ... folding and blocks RepZ translation (Asano & Mizobuchi, 1998). This system keeps RepZ levels low even...doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid...

3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998). FLPe’s performance...18614669. PubMed Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998....

4. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...Examples include the LOV domains that bind flavin (Buckley et al., 2015) or the UnaG protein that binds bilirubin...protein." (1998): 509-544. PubMed PMID: 9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence...

5. Viral Vectors 101: Systemic Capsids

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   Blog Post

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   Oct. 12, 2023, 1:15 p.m.

   ...et al., 2017, Vormstein-Schneider et al., 2020, Graybuck et al., 2021, Lawler et al., 2022). It can also...342. https://doi.org/10.1016/j.omtm.2022.07.010 Graybuck, L. T., Daigle, T. L., Sedeño-Cortés, A. E., Walker....org/10.1016/j.neulet.2017.11.049 Mich, J. K., Graybuck, L. T., Hess, E. E., Mahoney, J. T., Kojima, Y...

6. K. phaffii: Rising to the Occasion

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   Blog Post

   Published

   April 4, 2023, 1 p.m.

   ...yeast can also give more protein bang for your media buck - it grows at higher cell densities than most of...

7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...Ásgrímsdóttir ES, Rahimi K, Gill KP, Frandsen S, Hvolbøl Buchholdt S, Chen M, Kjems J, Febbraro F, Denham M (2018...

8. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...'s better for you to use cold block systems or buckets of ice. Try finding ways to intentionally set up...

9. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

11. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...and business training. The money is a drop in the bucket against the funds needed to build a flower that...

12. Plasmids 101: Degron Tags

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    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, et al. The dTAG system...

13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...of negative strand RNA viruses.   References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann...

14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...gene.  Validated gRNAs are a great bang for your buck – they have been used successfully in experiments...

15. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

16. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...also make them more attuned to the bang for their buck - make them demand more services based on what they're...

17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    .... 6(5): p. 568-72. PubMed PMID: 10802714. 41. Vierbuchen, T., et al., Direct conversion of fibroblasts...

18. Selecting Your Plasmid Purification Kit

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    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ... make sure you’re getting the best bang for your buck. If you’re unsure if a kit is a good option for ...