Addgene

We narrowed to 2,172 results for: c rel

Showing: 741 - 760 of 2172 results

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pBiT3.1-N_KIF21A:1337-1653

Plasmid

#211213

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW10:1-1061

Plasmid

#211207

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF1:1-1507

Plasmid

#211028

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_USP21:1-565

Plasmid

#211050

Purpose

Mammalian expression of full-length human USP21. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

USP21:M1-L565 (USP21 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW5:1-566

Plasmid

#211056

Purpose

Mammalian expression of full-length human FBXW5. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW5:M1-R566 (FBXW5 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF1:1038-1400

Plasmid

#211052

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_BOP1:1-746

Plasmid

#211004

Purpose

Mammalian expression of full-length human BOP1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

BOP1:M1-T746 (BOP1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-N_DCAF15:1-600

Plasmid

#210958

Purpose

Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF15:M1-L600 (DCAF15 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pOET3-N-TEV-Twin-Strep

Plasmid

#203323

Purpose

Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and Twin-strep-tag

Expression

Insect

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-mMcl1

Plasmid

#25714

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1 (Mcl1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
GFP-GID1

Plasmid

#37306

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GID1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T-1-mMcl-1

Plasmid

#25717

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (Mcl1 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-mMcl1(S140A)

Plasmid

#25383

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(S140A) (Mcl1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed serine 140 to alanine

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pHJ425

Plasmid

#20683

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; and change…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCPF101-pfQC-WT

Plasmid

#184877

Purpose

expresses soluble plasmodium falciparum QC protein with a histag

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-wt

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHOT-P2Y2R

Plasmid

#159108

Purpose

entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

Depositor

Fabio Mammano

Article

Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

Insert

P2Y2R

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx32D178Y-IRES-GCaMP6s

Plasmid

#216796

Purpose

Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

Depositor

Fabio Mammano

Article

Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

Insert

Cx32D178Y-IRES-GCaMP6s (GJB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

D178Y mutant form of Connexin 32

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-NanoLuc-BRAF-CAT

Plasmid

#194772

Purpose

Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)

Use

Tags

NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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