We narrowed to 30,515 results for: ide

1. jk67

   Plasmid

   #87013

   Purpose

   genomic Sindbis virus plasmid for use in SYNseq PLA screen

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Inserts

   CLIP-Nrx1B-4xλN(i)
   mCherry
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. IDP430

   Plasmid

   #87015

   Purpose

   genomic Sindbis virus plasmid for use in SYNseq PLA screen

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Inserts

   CLIP-Nrx1B-tdMS2(c)
   mCherry
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. IDP428

   Plasmid

   #87014

   Purpose

   genomic Sindbis virus plasmid for use in SYNseq PLA screen

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Inserts

   CLIP-Nrx1B-1xλN(c)
   mCherry
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM040 [Multiome Perturb-seq sgRNA expression vector]

   Plasmid

   #224898

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

   Depositor

   Thomas Norman

   Article

   Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNTI821

   Plasmid

   #216630

   Purpose

   Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID
   

   Use

   Sleeping beauty transposon system

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-StrepHA-SUMO2(Q87R)

   Plasmid

   #66868

   Purpose

   Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Q87R

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI823

   Plasmid

   #216632

   Purpose

   Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   Cal-ID (split site variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TTmiRc2

   Plasmid

   #25752

   Purpose

   Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TmiR_Luc-A

   Plasmid

   #25760

   Purpose

   Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TTGmiRc2

    Plasmid

    #25753

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEN_hUmiRc2

    Plasmid

    #25747

    Purpose

    Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEN_TTRmiRc2

    Plasmid

    #25754

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    dsRed2

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TGmiRc3

    Plasmid

    #25749

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBXPHM3-PCFT_NB

    Plasmid

    #165415

    Purpose

    Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT_NB (SLC46A1 Llama, Chicken)
    

    Use

    Tags

    His10-MBP-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    arabinose

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_TGmiR_Luc-A

    Plasmid

    #25765

    Purpose

    Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN_TRmiRc2

    Plasmid

    #25750

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    dsRed2

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEN_hU6miR-Luc-A

    Plasmid

    #25756

    Purpose

    Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

    Plasmid

    #182152

    Purpose

    To insert attB-rev attachment site at IDS in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    IDS-D1b-attB-rev pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

    Plasmid

    #182151

    Purpose

    To insert attB-rev attachment site at IDS in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    IDS-C2c-attBrev pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDDGFP-Leu2d_GgPCFT

    Plasmid

    #165414

    Purpose

    expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT (SLC46A1 Synthetic, Chicken)
    

    Use

    Tags

    GFP-His8

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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