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Plasmid#203767PurposeFor production of template RNA for use in PRINT, GFP under CBh promoterDepositorInsertPRINT template RNA for GFP
UseIvt templateTagsExpressionMutationPromoterCBhAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b
Plasmid#80612PurposepBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
TCARZ-SV40-GFP-PAmin-GF3-R4A22
Plasmid#203769PurposeFor production of template RNA for use in PRINT, GFP under SV40 promoterDepositorInsertPRINT template RNA for GFP
UseIvt templateTagsExpressionMutationPromoterSV40Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- PGK1prom-TIR1-FLAG
Plasmid#189724PurposeEnables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)DepositorInsertOsTIR1
UseTagsExpressionYeastMutationPromoterPGK1 promoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorInserteGFP L202 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
eGFP L138 gRNA
Plasmid#119133PurposegRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.DepositorInserteGFP L138 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Arp2/3_pBIG2abcd
Plasmid#173683PurposeInsect expression of the 7-subunit Human Arp2/3 complexDepositorInsertsArp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-hNCOA6:V5
Plasmid#63585PurposeA subunit of the Trithorax-related (Trr) methyltransferase complex.DepositorInsertNCOA6 (NCOA6 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt_hNANOG
Plasmid#112639PurposePlasmid for PCR amplification of hNANOG DNA template for in vitro transcriptionDepositorInserthNANOG (NANOG Human)
UsePcr templateTagsExpressionMutationPromoterT7Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpyTag-GlpG
Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
UseTags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only