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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,030 results for: pET

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Showing: 741 - 760 of 10030 results

  1. p028g34-TEVs

    Plasmid
    #249633
    Purpose
    Clone the cleavage site for TEV protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    Recognition site for TEV protease
    Expression
    Bacterial
    Available since
    April 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    249633-plasmid-map-sequence-id-506112

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  2. p028g13-Gb1

    Plasmid
    #249632
    Purpose
    Clone 8xHis-residues in tandem with Gb1 polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide
    Expression
    Bacterial
    Available since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249632-plasmid-map-sequence-id-504978

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  3. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  4. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  5. pN23-LIC-FLAG-STREP

    Plasmid
    #182508
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    STREP-FLAG-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182508-plasmid-map-sequence-id-361393

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  6. pNYCOMPS-C-Lic-His-FLAG

    Plasmid
    #182505
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    TEV-FLAG-10x His
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182505-plasmid-map-sequence-id-361391

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  7. pNYCOMPS-N-Lic-His-FLAG

    Plasmid
    #182506
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    10xHis-FLAG-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182506-plasmid-map-sequence-id-358073

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  11. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid
    #166415
    Purpose
    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Publication
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Acytostelium subglobosum PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166415-plasmid-map-sequence-id-329462

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  14. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Plasmid
    #166418
    Purpose
    Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Publication
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Drosophila melanogaster PRL (Prl Fly)
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166418-plasmid-map-sequence-id-329456

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  17. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201819
    Purpose
    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter
    Depositor
    Christopher Richie
    Publication
    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Expression
    Mammalian
    Promoter
    pOTTC1751
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201819-plasmid-map-sequence-id-399602

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  18. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

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  20. Sc GST-P-Pol32-pLANT(2b)/RIL

    Plasmid
    #257903
    Purpose
    Over-express Sc GST/P-Pol32 in E. coli. To be used in conjunction with Sc Pol31-pET(16b) (no tag) (Addgene #257902) to over-express Sc [Pol31 + GST/P-Pol32] complex in E. coli.
    Depositor
    Michael O'Donnell
    Insert
    Sc Pol32
    Tags
    GST tag followed by PreScission Protease site
    Expression
    Bacterial
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    257903-plasmid-map-sequence-id-516870

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Showing: 741 - 760 of 10030 results