We narrowed to 15,120 results for: pET

1. pET28a-DDX43

   Plasmid

   #134574

   Purpose

   Express human DDX43 protein (WT, full-length) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43 (DDX43 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length WT

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11a-Z-NGFP

   Plasmid

   #52734

   Purpose

   IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   NGFP+Z-fusion
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Tags

   his-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-AldoA

   Plasmid

   #224925

   Purpose

   Inducible expression of human aldolase A

   Depositor

   Liskin Swint-Kruse

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   Aldolase A (ALDOA Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-irp3 WT

   Plasmid

   #87106

   Purpose

   expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

   Insert

   irp3 WT
   

   Tags

   his-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-5'TwStrep

   Plasmid

   #208620

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-Opt13

   Plasmid

   #61698

   Purpose

   Expresses PTDH mutant Opt13

   Depositor

   Huimin Zhao

   Article

   McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

   Insert

   Opt13
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11d-hUba2

   Plasmid

   #53136

   Purpose

   E.coli expression of full length untagged human Uba2 (SAE2)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   UBA2 (UBA2 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4CyPet-MAMM

    Plasmid

    #14033

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    CyPet
    

    Expression

    Mammalian

    Mutation

    Mammalian optimized CyPet

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-53-RtcA

    Plasmid

    #107876

    Purpose

    Expresses E. coli RtcA in bacterial expression cells

    Depositor

    Jay Hesselberth

    Insert

    E. coli RtcA (rtcA Escherichia coli)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET3a-Yuh1

    Plasmid

    #18895

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pET-X(5010)B1

    Plasmid

    #168019

    Purpose

    Expresses X(5010)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(5010)B1-6xHis
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET16_Caspase-4_p10

    Plasmid

    #214308

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_Caspase-4_p20_C258A

    Plasmid

    #214307

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    C258A

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-PHGDH

    Plasmid

    #74113

    Purpose

    Expression of N-terminal His-tagged

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His-tagged

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET41c-PhTET2

    Plasmid

    #182428

    Purpose

    Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

    Depositor

    Bruno Franzetti

    Article

    Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

    Insert

    Tetrahedral aminopeptidase TET2 from P. hirokoshii
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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